Homeopathic remedy Psorinum 6 × triggers apoptosis signals in human lung cancer cells.

Introduction

Great efforts have been made to successfully combat
various types of cancer including improved surgical
tools, radiotherapy and chemotherapy. However,
such interventions, due to financial limitations, are
not available to huge populations, particularly in the
developing world. Further, the number of cancer cases

has increased so much so that many cancer patients are
unable to obtain mainstream cancer treatments because of
inadequate medical infrastructure, in addition to nancial
constraints. Additionally, the prognosis of some forms

of cancer, like cancers of liver, gall bladder, pancreas, and
stomach, is still poor. Elderly cancer patients may not

tolerate conventional cancer treatments because of old
age-related problems, including lack of strength to cope

up with chemotherapy and/or radiotherapy. Therefore, in
recent years, there has been a serious search for efcient
complementary and alternative medicines (CAMs) which
could render additional benets, particularly when used in
combination with the mainstream Western medicines.

Psorinum therapy is one CAM practice, whose promising
results have been advertised by a group of Indian scientists
and clinician. However, these studies are based on

single-armed experimental design, without any control,
weakening their conclusions. Thus they do not provide
the convincing evidence needed to recommend the safe
use of Psorinum treatment in human subjects. In fact, in
the homeopathic regimen, it has been suggested that quite
a few remedies have considerable anticancer effects.

As a result, alternative cancer treatments have gained
considerable importance in oncology throughout the
world. Among several homeopathic treatment protocols
now being used in India , with varying degree of success,

Psorinum therapy is believed to treat several forms
of cancer successfully, enabling the patients to survive for
several years with improved quality of life.

cancer cells.jpg
Mondal J1, Samadder A1,2, Khuda-Bukhsh AR1.

OBJECTIVE: To provide in vitro evidence of Psorinum treatment against cancer cells in a controlled study.

METHODS: Effects of homeopathic Psorinum 6× on cell viability were initially determined in several cancer cell lines, including A549, HepG2 and MCF-7, using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and an ethanol 6× control. The cell line that exhibited highest inhibition was selected and used in the following experiments. A range of Psorinum 6× doses was used to explore treatment effects on cell cycle arrest, cell death (apoptosis), generation of reactive oxygen species (ROS) and change in mitochondrial membrane potential (MMP) using flow cytometry and fluorescence microscopy, respectively. Expression of several signal proteins related to apoptosis and cell survival were quantified with Western blotting and confocal microscopy. Further, circular dichroism (CD) spectroscopy was used to determine possible drug-DNA interactions, as well as the induction of conformational changes.

RESULTS: Treatment of cancer cell lines with Psorinum showed greater anticancer effects in A549 cells than in others. In A549 cells Psorinum treatment inhibited cell proliferation at 24 h after treatment, and arrested cell cycle at sub-G1 stage. It also induced ROS generation, MMP depolarization, morphological changes and DNA damage, as well as externalization of phosphatidyl serine. Further, increases in p53 expression, Bax expression, cytochrome c release, along with reduction of Bcl-2 level and caspase-3 activation were observed after Psorinum 6× treatment, which eventually drove A549 cells towards the mitochondria-mediated caspase-3-dependent pathway. CD spectroscopy revealed direct interaction of Psorinum with DNA, using calf thymus-DNA as target.

CONCLUSION: Psorinum 6× triggered apoptosis in A549 cells via both up- and down-regulations of relevant signal proteins, including p53, caspase-3, Bax and Bcl-2.

Courtesy:   http://www.ncbi.nlm.nih.gov/pubmed/26988436

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